Journal
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
Volume 497, Issue 1-2, Pages 139-145Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S1383-5718(01)00251-0
Keywords
chlorophyll; antigenotoxic; cell culture; micronucleus test; chlorophyllin
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Chlorophyll and its derivatives are examples of plant compounds (purified and/or extracted) which appear to protect DNA from damage caused by chemical or physical agents, although some studies have identified clastogenic activity of these compounds. This study was carried out to assess the genotoxic activity of chlorophyll-a (Chl-a), -b (Chl-b) and chlorophyllin (Chl) and their antigenotoxic activity against the DNA damage induced by methyl methanesulphonate (MMS) under conditions of simultaneous, pre-, post-treatment, and simultaneous treatment after pre-incubation of the chemical with MMS. The micronucleus (MN) test was used in binucleated cells (induced by cytocalasin-B) of a mammalian cell line (V79). The three concentrations of Chl-a, Chl-b or Chi (0.1375, 0.275, 0.53 muM) were not genotoxic and the genotoxic action of MMS (400 muM) decreased (74-117%) under all treatment conditions. The results showed that there was no significant difference among the treatment types, the concentration or the nature of chlorophyll used. The data obtained suggest that Chl-a, Chl-b and Chl when associated with the DNA damaging agent, MMS, may protect the DNA by desgenotoxic action and/or by bio-antigenotoxic mechanisms, with the similar efficiency. (C) 2001 Elsevier Science B.V. All rights reserved.
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