Journal
NEUROREPORT
Volume 12, Issue 15, Pages 3401-3405Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00001756-200110290-00050
Keywords
chronic constriction injury; dorsal root ganglia; neuropathic pain; ordered differential display-polymerase chain reaction
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Ordered differential display PCR was used to identify differentially expressed genes in rat dorsal root ganglia at 7 days following chronic constriction injury (CCI) of the sciatic nerve. Fourteen differentially displayed cDNA bands were isolated, cloned and verified by RT-PCR. The four mRNAs were increased, which included mRNAs encoding heat shock protein 27, fatty acid binding protein, apolipoprotein D and one novel gene. Six down-regulated clones were microtubule-associated protein IB, protein tyrosine phosphatase alpha, Kv1.2 channel, myelin protein SR13, medium-sized neurofilament protein, and one novel gene. Our results show that many differentially regulated genes after CCI may play a role in nerve degeneration and/or regeneration and provide a molecular framework for understanding the peripheral mechanism underlying neuropathic pain. NeuroReport 12:3401-3405 (C) 2001 Lippincott Williams & Wilkins.
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