Journal
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
Volume 17, Issue 1-2, Pages 75-78Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0928-4931(01)00340-X
Keywords
mechanical stretch; myogenic cells; myogenic regulatory factors
Categories
Ask authors/readers for more resources
The purpose of this study was to examine whether mechanical stretch alters the expression of myogenic regulatory factors in cultured myogenic satellite cells. C2Cl2 myogenic satellite cells were plated on collagenized silastic membrane and cultured in growth medium and stretched with 20% elongation for 24 h. The expressions of myogenic regulatory factors including MyoD, myogenin, myocyte nuclear factor (MNF)-alpha and -beta were determined by semiquantitive reverse transcriptional-polymerase chain reaction (RT-PCR) analysis, The expressions of MyoD and MNF-alpha were decreased in stretched C2Cl2 as compared to control condition. The expressions of myogenin and MNF-beta were not detected. These results demonstrate that mechanical stretch inhibits differentiation of C2Cl2 myogenic satellite cells to myotube in the cultured condition. (C) 2001 Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available