Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 257, Issue 1-2, Pages 213-220Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0022-1759(01)00467-7
Keywords
inflammation; leukocytes; chemokines; cytokines; cantharidin
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A skin blister technique is described which allows the investigation of acute inflammation in humans in vivo. Filter paper discs are placed on the skin, impregnated with cantharidin and covered with impermeable film held by adhesive tape. The assembly is easily applied, unobtrusive, stable and may be worn during normal activities. The blister formed at 24 h contains approximately 5 X 10(5)-5 X 10(6) cells, predominantly neutrophils and macrophages. Inflammatory cytokines and chemotactic factors are detectable in the blister fluid. The technique is useful for characterizing the acute inflammatory response in health and disease. (C) 2001 Elsevier Science B.V. All rights reserved.
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