Journal
EMBO JOURNAL
Volume 20, Issue 21, Pages 6115-6126Publisher
WILEY
DOI: 10.1093/emboj/20.21.6115
Keywords
checkpoint; initiation; replication; Schizosaccharomyces pombe; timing
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Funding
- NCI NIH HHS [CA84302] Funding Source: Medline
- NIGMS NIH HHS [GM49294] Funding Source: Medline
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Here we report the first characterization of replication timing and its regulation in the fission yeast Schizosaccharomyces pombe. We used three different synchronization methods: centrifugal elutriation, cdc10 temperature-shift and release, and starvation for deoxyribonucleoside triphosphates (dNTPs) by treatment with hydroxyurea (HU) followed by removal of HU, to study the times when specific autonomously replicating sequence elements (ARS elements; potential replication origins) replicate during S phase. We found that individual ARS elements replicate at characteristic times, some early and some late, independently of synchronization method. In wild-type cells treated with HU, early ARS elements replicated but late ones did not. However, in HU-treated mutant cells lacking the Rad3 (similar to human ATR and ATM) or Cds1 (similar to human CHK2) checkpoint kinase, both early and late ARS elements were able to replicate. Thus under conditions of dNTP starvation the Rad3 and Cds1 kinases are needed to suppress the replication of normally late-replicating regions.
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