Journal
PHYTOCHEMISTRY
Volume 58, Issue 6, Pages 897-904Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0031-9422(01)00335-1
Keywords
woad; Isatis tinctoria; Brassicaceae; indigo; indirubin; isoindirubin; isatan; indican
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Indole is presumably a product of indole-3-glycerol phosphate catabolism in Isatis tinetoria. It is oxidized into indoxyl and stored in young leaves Lis indigo precursor, Further oxidation and dimerization of indoxyl produces indigoid pigments. In this work, we describe an HPLC method dedicated to the identification and quantification of indigoid pigments (indigo, indirubin, isoindigo and isoindirubin) and indigo precursors produced in I. tinetoria (Woad). This work. carried out with two cultivars of I. tinetoria. has confirmed that the quantity of indigo precursors is dependent on the species and the harvest period. In addition we have shown for the first time that young leaves of I. tinetoria, harvested in June contained a new indigo precursor in addition to isatan B (indoxyl-5-ketogluconate) and indican (indoxyl-beta -D-glucoside). We suggest the name isatan C for this new indigo precursor in I. tinetoria. Its chemical characteristics point to an dioxindole ester with PM of 395. We have shown that isatan C reacts with isatan B increasing the red pigment production. (C) 2001 Elsevier Science Ltd. All rights reserved.
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