Journal
PLANT CELL
Volume 13, Issue 11, Pages 2513-2523Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.13.11.2513
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Funding
- NIGMS NIH HHS [R01 GM60396-01, R01 GM060396] Funding Source: Medline
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The hormone abscisic acid (ABA) regulates stress responses and developmental processes in plants. Calcium-permeable channels activated by reactive oxygen species (ROS) have been shown recently to function in the ABA signaling network in Arabidopsis guard cells. Here, we report that ABA activation of these 1(ca) Ca2+ channels requires the presence of NAD(P)H in the cytosol. The protein phosphatase 2C (PP2C) mutant abi1-1 disrupted ABA activation of 1(ca). channels. Moreover, in ab1-1, ABA did not induce ROS production. Consistent with these findings, in abi1-1, H2O2 activation of 1(ca). channels and H2O2-induced stomatal closing were not disrupted, suggesting that abi1-1 impairs ABA signaling between ABA reception and ROS production. The abi2-1 mutation, which lies in a distinct PP2C gene, also disrupted ABA activation of 1(ca). However, in contrast to abi1-1, abi2-1 impaired both H2O2 activation of 1(ca) and H2O2-induced stomatal closing. Furthermore, ABA elicited ROS production in abi2-1. These data suggest a model with the following sequence of events in early ABA signal transduction. ABA, abi1-1, NAD(P)H-dependent ROS production, abi2-1, 1(ca) Ca2+ channel activation followed by stomatal closing.
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