Journal
AVIAN DISEASES
Volume 57, Issue 2, Pages 273-279Publisher
AMER ASSOC AVIAN PATHOLOGISTS
DOI: 10.1637/10326-081512-Reg.1
Keywords
Escherichia coli; avian pathogenic Escherichia coli; chicken; turkey; PCR; avian; phenotypic; vaccine; genotypic
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A candidate live vaccine for avian pathogenic Escherichia coli (APEC) was constructed from a virulent field APEC O78 strain by mutation of the aroA gene. The mutant was highly similar to the parent wild-type strain in respect of colony morphology, motility, growth in suspension, hemagglutination, Congo Red binding, HEp-2 cell adhesion, and the elaboration of surface antigens type 1 fimbriae and flagella, although production of curli fimbriae was reduced marginally. The mutant proved avirulent when inoculated into 1-day-old chicks by spray application and when presented again in the drinking water at 7 days of age. Chickens and turkeys vaccinated with an O78 aroA mutant were protected against a challenge at 6 wk of age by virulent APEC strains.
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