Journal
HYPERTENSION
Volume 38, Issue 5, Pages 1107-1111Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/hy1101.093423
Keywords
deoxycorticosterone acetate; NADH/NADPH; mineralocorticoids; hypertension, mineralocorticoid
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Funding
- NHLBI NIH HHS [HL-18575] Funding Source: Medline
- NIGMS NIH HHS [2-T32-GME0322-11] Funding Source: Medline
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We previously reported increased aortic reactive oxygen species (ROS) production in mineralocorticoid (deoxycorticosterone acetate [DOCA]-salt) hypertensive rats. In the present study, we tested the hypothesis that NADH/NADPH oxidase is responsible for increased ROS production, namely superoxide (O-2(-)), in aorta from the DOCA-salt rat. Treatment of aortic rings from DOCA-salt rats with the NO synthase inhibitor N-nitro-L-arginine and the xanthine oxidase inhibitor allopurinol did not significantly change O-2(-) production. Furthermore, de-endothelialization of aorta from DOCA-salt rats did not affect O-2(-) production compared with that of sham-operated rats. Thus, xanthine oxidase and uncoupled endothelial NO synthase were not responsible for increased O-2(-) production in the DOCA-salt rats. In contrast, treatment with the NADPH oxidase inhibitor apocynin significantly decreased O-2(-) production in aortic rings from DOCA-salt rats compared with sham-operated rats. Moreover, long-term administration of apocynin (in drinking water, 1.5 mmol/L, 28 days) to DOCA-salt rats significantly decreased systolic blood pressure compared with that of rats treated with DOCA-salt alone. Furthermore, O-2(-) production in aortic rings from DOCA-salt rats treated with apocynin for 28 days was reduced compared with that of untreated DOCA-salt rats. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis demonstrated that DOCA-salt rats have significantly greater mRNA levels of the NADPH oxidase subunit p22phox than do sham-operated rats. These findings suggest that NADPH oxidase is increased and is responsible for increased O-2(-) production and possibly contributes to increased blood pressure in the DOCA-salt hypertensive rat.
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