Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 49, Issue 11, Pages 5589-5594Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf010621r
Keywords
carbazole; Murraya koenigii; curry leaf; antioxidant; oil stability index (OSI); 1-1-diphenyl-2-picrylhydrazyl (DPPH)
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The antioxidative properties of the leaves extracts of Murraya koenigii using different solvents were evaluated based on the oil stability index (OSI) together with their radical scavenging ability against 1-1-diphenyl-2-picrylhydrazyl (DPPH). The methylene chloride (CH2Cl2) extract and the ethyl acetate (EtOAc) soluble fraction of the 70% acetone extract significantly prolonged the OSI values comparable to those of alpha -tocopherol and BHT. Five carbazole alkaloids were isolated from the CH2Cl2 extract and their structures were identified to be euchrestine B (1), bismurrayafoline E (2), mahanine (3), mahanimbicine (4), and mahanimbine (5) based on H-1 and C-13 NMR and mass (MS) spectral data. The OSI value of carbazoles at 110 degreesC decreased in the order 1 and 3 > alpha -tocopherol > BHT > 2 > 4, 5 and control. It is' assumed that compounds 1 and 3 contributed to the high OSI. value of the CH2Cl2 extract of M. koenigii. The DPPH radical scavenging activity for these carbazoles-was in the order ascorbic acid > 2 > 1, 3 and alpha -tocopherol > BHT > 4 and 5.
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