Determination of cyclopiazonic acid in cheese samples using solid-phase microextraction and high performance liquid chromatography

Solid phase microextraction (SPME), using a Carbowax/Templated Resin fiber, was optimized for the determination of the mycotoxin cyclopiazonic acid (CPA) and interfaced with HPLC-UV/DAD. All the parameters influencing SPME-HPLC, including extraction time and temperature, pH, ionic strength and desorption conditions, have been carefully examined. The method was successfully applied to the analysis of white surface cheese samples. SPME was capable of a selective extraction of CPA after a short sonication step in methanol. The whole extraction gave high recovery yields and was simpler and quicker than any other existing procedure for CPA extraction from cheese. A detection limit in cheese of 7 ppb was obtained. (C) 2001 Elsevier Science Ltd. All rights reserved.