Journal
AUTOPHAGY
Volume 6, Issue 5, Pages 642-644Publisher
LANDES BIOSCIENCE
DOI: 10.4161/auto.6.5.12092
Keywords
lysosome; protein degradation; protein targeting; stress; vacuole
Categories
Funding
- NIGMS NIH HHS [R01 GM053396] Funding Source: Medline
Ask authors/readers for more resources
The transparency, external development and simple drug administration of zebrafish embryos makes them a useful model for studying autophagy during embryonic development in vivo. Cloning of zebrafish lc3 and generation of a transgenic GFP-Lc3 fish line provide excellent tools to monitor autophagy in this organism. 1 This protocol discusses several convenient autophagy assays in zebrafish, including immunoblotting of Lc3 lipidation, microscopy imaging of GFP-Lc3 and lysosomal staining.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
