Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 76, Issue 3, Pages 187-192Publisher
WILEY-BLACKWELL
DOI: 10.1002/bit.10007
Keywords
minimal cell; cell model; kinetic scaling; cell composition
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A model of a minimal cell would be a valuable tool in identifying the organizing principles that relate the static sequence information of the genome to the dynamic functioning of the living cell. Our approach for developing a minimal cell model is to first generalize an existing model of Escherichia coli by expressing reaction rates as ratios to a set of reference parameters. This generalized model is a prototype minimal cell model that will be developed by adding detail to explicitly include each chemical species. We tested the concept of a generalized model by testing the effect of scaling all enzyme-catalyzed reactions in the E. coli model. The scaling has little effect on cellular function for a wide range of kinetic ratios, where the kinetic ratio is defined as the rate of all enzyme-catalyzed reactions in a given model relative to those in the E. coli model. (C) 2001 John Wiley & Sons, Inc.
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