Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 49, Issue 11, Pages 5270-5272Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf0106751
Keywords
bleaching herbicides; competitive inhibitor; enzyme kinetics; norflurazon; phytoene desaturase
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Cofactor requirement was determined for the heterologous expressed phytoene desaturases from the cyanobacterium Synechococcus and the higher plant Gentiana lutea. The cyanobacterial enzyme is dependent on either NAD(P) or plastoquinone, whereas only quinones such as plastoquinone can function as a cofactor for the phytoene desaturase from G. lutea. Enzyme kinetic studies were carried out to determine a possible competition between the cofactors and the bleaching herbicide norflurazon. For the Synechococcus enzyme, competition between norflurazon and NADP, as well as plastoquinone, could be demonstrated. The K-m values for these cofactors, were 6.6 mM and 0.23 muM, respectively. Inhibition of the phytoene desaturase from G. lutea by norflurazon was also competitive with respect to plastoquinone. The K-m values of both enzymes for plastoquinone were very close.
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