4.8 Article

Macroautophagy-dependent, intralysosomal cleavage of a betaine homocysteine methyltransferase fusion protein requires stable multimerization

Journal

AUTOPHAGY
Volume 4, Issue 2, Pages 185-194

Publisher

LANDES BIOSCIENCE
DOI: 10.4161/auto.5275

Keywords

macroautophagy; autophagosomes; betaine homocysteine methyltransferase; multimerization; cargo-based assay

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BHMT, betaine homocysteine methyltransferase; 3-MA, 3-methyladenine; GST, glutathione S-transferase; PNS, post-nuclear supernatant; HSS, high-speed supernatant; LMF, light membrane fraction; DMEM, Dulbecco's Modified Eagle's Medium, TCA, trichloroacetic acid; GFP, green fluorescent protein; FCS, fetal calf serum; IRES, internal ribosomal entry site; HA, hemagglutinin; LAMP-1, lysosomal-associated membrane protein 1; hULK1, human unc-51-like kinase 1

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