Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 276, Issue 44, Pages 40373-40376Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.C100505200
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- NIDDK NIH HHS [DK54345, R01 DK054345-02] Funding Source: Medline
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Transcriptional regulation is commonly associated with local levels of histone acetylation, which controls chromatin structure at specific genes or within contiguous chromosomal domains. Less well understood are the higher order determinants of histone acetylation. The transcription factor, CCAAT/enhancer-binding protein alpha (C/EBP alpha), concentrates at one higher order structure, the peri-centromeric chromatin, and regulates differentiation in many cell types, including pituitary cells. We used quantitative fluorescence microscopy to show that immunostained acetylated histone H3 is relatively absent from peri-centromeric domains visible as large structures in mouse pituitary progenitor GHFT1-5 cells. GHFT1-5 cells do not contain C/EBP alpha. We observed that expression of C/EBP alpha in GHFT1-5 cells leads to an increased level of acetylated historic H3, but not acetylated historic H4, at the peri-centromeric domains. Only transcriptionally active forms of C/EBP alpha altered histone acetylation at the peri-centromeric domain. The altered state of histone acetylation at large intranuclear domains may complement, counteract, or supercede the more gene-local activities of other transcription factors to coordinate C/EBP alpha -induced cellular differentiation.
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