Journal
FEMS MICROBIOLOGY LETTERS
Volume 204, Issue 2, Pages 323-328Publisher
ELSEVIER SCIENCE BV
DOI: 10.1111/j.1574-6968.2001.tb10905.x
Keywords
adhesion; gene disruption; medical mycology; mutagenesis; Candida albicans
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Gene disruptions in the diploid opportunistic human fungal pathogen Candida albicans are usually created using multiple rounds of targeted integration called the 'ura-blaster' method. Resulting heterozygous and homozygous null mutants can be auxotrophic (Ura-) or prototrophic (Ural) for uracil biosynthesis. Here we demonstrate that the Ura-status of otherwise isogenic mutants affected the adhesion of C albicans. Moreover the effect of Ura-status on adhesion was also dependent on the null mutant background, the nature of the underlying surface and the carbon source for growth. Therefore the Ura-status is not neutral in determining adhesive properties of C albicans mutants that are generated via the ura-blaster protocol. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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