Journal
ANALYST
Volume 140, Issue 16, Pages 5518-5525Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5an00874c
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Funding
- Natural Sciences and Engineering Research Council (NSERC) of Canada
- Natural Science Foundation of China [61174010]
- Shanxi provincial government (100-talents program)
- Shanxi province [2012081043]
- Scholarship Council of China [2013-038]
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In this paper, we present a smartphone-readable barcode assay for the qualitative detection of methyl parathion residues, a toxic organophosphorus pesticide that is popularly used in agriculture worldwide. The detection principle is based on the irreversible inhibition of the enzymatic activity of acetylcholinesterase (AchE) by methyl parathion; AchE catalytically hydrolyzes acetylthiocholine iodine to thiocholine that in turn dissociates dithiobis-nitrobenzoate to produce a yellow product (deprotonated thio-nitrobenzoate). The yellow intensity of the product was confirmed to be inversely dependent on the concentration of the pesticide. We have designed a barcode-formatted assay chip by using a PDMS (polydimethylsiloxane) channel plate (as the reaction reservoir), situated under a printed partial barcode, to complete the whole barcode such that it can be directly read by a barcode scanning app installed on a smartphone. The app is able to qualitatively present the result of the pesticide test; the absence or a low concentration of methyl parathion results in the barcode reading as -, identifying the test as negative for pesticides. Upon obtaining a positive result (the app reads a + character), the captured image can be further analyzed to quantitate the methyl parathion concentration in the sample. Besides the portability and simplicity, this mobile-app based colorimetric barcode assay compares favorably with the standard spectrophotometric method.
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