Journal
ANALYTICAL CHEMISTRY
Volume 73, Issue 22, Pages 5525-5531Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac010762s
Keywords
-
Categories
Funding
- NIGMS NIH HHS [R01-GM59622-02] Funding Source: Medline
Ask authors/readers for more resources
Microfluidic channels fabricated from poly(dimethylsiloxane) (PDMS) are employed in surface plasmon resonance imaging experiments for the detection of DNA and RNA adsorption onto chemically modified gold surfaces. The PDMS microchannels are used to (i) fabricate 1-D single-stranded DNA (ssDNA) line arrays that are used in SPR imaging experiments of oligonucleotide hybridization adsorption and (ii) create 2-D DNA hybridization arrays in which a second set of PDMS microchannels are placed perpendicular to a 1-D line array in order to deliver target oligonucleotide solutions. In the 1-D line array experiments, the total sample volume is 500 muL; in the 2-D DNA array experiments, this volume is reduced to I muL. As a demonstration of the utility of these microfluidic arrays, a 2-D DNA array is used to detect a 20-fmol sample of in vitro transcribed RNA from the uidA gene of a transgenic Arabidopsis thaliana plant. It is also shown that this array fabrication method can be used for fluorescence measurements on chemically modified gold surfaces.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available