Journal
NUCLEIC ACIDS RESEARCH
Volume 29, Issue 22, Pages 4581-4588Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/29.22.4581
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An RNA degrading, high molecular weight complex was purified from Rhodobacter capsulatus. N-terminal sequencing, glycerol-gradient centrifugation and immunoaffinity purification as well as functional assays were used to determine the physical and biochemical characteristics of the complex. The complex comprises RNase E and two DEAD-box RNA helicases of 74 and 65 kDa, respectively. Most surprisingly, the transcription termination factor Rho is a major, firmly associated component of the degradosome.
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