Integrated graphical analysis of protein sequence features predicted from sequence composition

Several protein sequence analysis algorithms are based on properties of amino acid composition and repetitiveness. These include methods for prediction of secondary structure elements, coiled-coils, transmembrane segments or signal peptides, and for assignment of low-complexity, nonglobular, or intrinsically unstructured regions. The quality of such analyses can be greatly enhanced by graphical software tools that present predicted sequence features together in context and allow judgment to be focused simultaneously on several different types of supporting information. For these purposes, we describe the SFINX package, which allows many different sets of segmental or continuous-curve sequence feature data, generated by individual external programs, to be viewed in combination alongside a sequence dot-plot or a multiple alignment of database matches. The implementation is currently based on extensions to the graphical viewers Dotter and Blixem and scripts that convert data from external programs to a simple generic data definition format called SFS. We describe applications in which dot-plots and flanking database matches provide valuable contextual information for analyses based on compositional and repetitive sequence features. The system is also useful for comparing results from algorithms run with a range of parameters to determine appropriate values for defaults or cutoffs for large-scale genomic analyses. (C) 2001 Wiley-Liss, Inc.