Journal
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
Volume 18, Issue 2, Pages 172-183Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10286020.2015.1075005
Keywords
synergistic antibiotic activity; Saraca asoca; Caesalpiniaceae; beta-lactam antibiotics; in silico mechanism; (+)-pinitol
Funding
- DBT, New Delhi
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Saraca asoca bark has been used in the Ayurvedic system of medicine for female urino-genital disorders. We have recently reported the isolation and characterization of several compounds as markers to develop HPLC profiling of its methanol and aqueous methanol extracts. Now, a HPLC-PDA inactive compound, (+)-pinitol has been isolated and characterized from the bark of this medicinally important tree. Pinitol is a well known bioactive compound for a variety of biological activities, including hypoglycemic and anti-inflammatory activities. A process for the isolation of relatively good concentration of (+)-pinitol from S. asoca bark has been developed and its in vitro anti TNF-alpha and anti-inflammatory activities against carragenan-induced edema confirmed. While conducting experiments on the possible agonistic activity, it was found that (+)-pinitol showed up to eight fold reduction in the doses of beta-lactam antibiotics. The mechanism of its agonistic activity was studied by docking experiments which showed that different conformations of (+)-pinitol and antibiotics were actually in the same binding site with no significant change in the binding energy. These docking simulations, thus predict the possible binding mode of studied compounds and probable reason behind the synergistic effect of (+)-pinitol along with beta-lactam antibiotics.
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