SELEX for tubulin affords specific T-rich DNA aptamers

We succeeded in acquiring two DNA aptamers that selectively recognize tubulin by the SELEX method. A pool of single-stranded oligo-DNAs including a random region of 59 nucleotides was screened by SELEX for tubulin purified from calf-brain as a target. After 20 repetitions of selection round, the library converged on specific T-rich sequences. The binding activity of T-rich clones was analyzed by the SPR sensor to determine their dissociation constants to be in the order of 10 muM. (C) 2000 Elsevier Science Ltd. All rights reserved. (C) 2001 Elsevier Science Ltd. All rights reserved.