Journal
JOURNAL OF CELL BIOLOGY
Volume 155, Issue 5, Pages 739-745Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200109026
Keywords
-
Categories
Ask authors/readers for more resources
The axon initial segment is an excitable membrane highly enriched in voltage-gated sodium channels that integrates neuronal inputs and initiates action potentials. This study identifies Na(v)1.6 as the voltage-gated sodium channel isoform at mature Purkinje neuron initial segments and reports an essential role for ankyrin-G in coordinating the physiological assembly of Na(v)1.6, beta IV spectrin, and the Ll cell adhesion molecules (L1 CAMs) neurofascin and NrCAM at initial segments of cerebellar Purkinje neurons. Ankyrin-G and PIV spectrin appear at axon initial segments by postnatal day 2, whereas L1 CAMs and Na(v)1.6 are not fully assembled at continuous high density along axon initial segments until postnatal day 9. L1 CAMs and Na(v)1.6 therefore do not initiate protein assembly at initial segments. beta IV spectrin, Na(v)1.6, and L1 CAMs are not clustered in adult Purkinje neuron initial segments of mice lacking cerebellar ankyrin-G. These results support the conclusion that ankyrin-G coordinates the physiological assembly of a protein complex containing transmembrane adhesion molecules, voltage-gated sodium channels, and the spectrin membrane skeleton at axon initial segments.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available