Journal
FEBS LETTERS
Volume 509, Issue 1, Pages 77-80Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(01)03135-0
Keywords
desaturase; elongase; polyunsaturated ratty acid; essential fatty acid biosynthesis
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Yeast co-expressing human elongase and desaturase genes were used to investigate whether the same desaturase gene encodes an enzyme able to desaturate n-3 and n-6 fatty acids with the same or different carbon chain length. The results clearly demonstrated that a single human Delta5 desaturase is active on 20:3n-6 and 20:4n-3. Endogenous Delta6 desaturase substrates were generated by providing to the yeast radiolabelled 20:4n-6 or 20:5n-3 which, through two sequential elongations, produced 24:4n-6 and 24:5n-3, respectively. Overall, our data suggest that a single human Delta6 desaturase is active on 18:2n-6, 18:3n-3, 24:4n-6 and 24:5n-3. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
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