Journal
PHYTOCHEMISTRY
Volume 58, Issue 8, Pages 1297-1303Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0031-9422(01)00344-2
Keywords
Pseudomonas fluorescens; antibiotic; sporegermination; germ tube growth
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Funding
- NIGMS NIH HHS [R15 GM122030] Funding Source: Medline
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Pseudomonas fluorescens strain G308 isolated from barley leaves produces a novel antibiotic substance that was purified by preparative TLC and HPLC and identified as N-mercapto-4-formylcarbostyril (Cbs) by LC/DAD, IR, LC-ES+/MS, LC-ES-/MS, GC-EI/MS, LC-HRES+ /MS, mass isotope ratios analysis, H-1 NMR and C-13 NMR analysis. The purified new antibiotic compound is effective against many phytopathogenic fungi in vitro. The compound inhibited Lit 25 ppm spore germination and germ tube growth of the following fungi, Fusarium oxysporum f. sp. lycopersici, Fusarium culmorum, Cladosporium cucumerinum and Colletotrichum lagenarium, At concentrations up to 125 ppm, the compound did not interfere with release of zoospores from sporangia and germination of encysted zoospores of Phytophthora infestans. (C) 2001 Elsevier Science Ltd. All rights reserved.
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