Journal
JOURNAL OF VIROLOGY
Volume 75, Issue 24, Pages 12431-12438Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.75.24.12431-12438.2001
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Funding
- NIAID NIH HHS [R01 AI043000, AI43000] Funding Source: Medline
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To facilitate detection of glycoprotein K (gK) specified by herpes simplex virus, a 12-amino-acid epitope tag was inserted within gK domain III. Recombinant virus gKprotC-DIII, expressing the tagged gK, was isolated. This virus formed wild-type plaques and replicated as efficiently as the wild-type KOS virus in Vero cells. Anti-protein C MAb detected high-mannose and Golgi complex-dependent glycosylated gK within cells as well as on purified virions. The gK-null virus Delta gK (gK(-/-)) entered Vero cells substantially more slowly than the wild-type KOS (gK(+/+)), while Delta gK virus grown in complementing VK302 cells (gK(-/+)) entered with entry kinetics similar to those of the KOS virus.
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