Journal
INTERNATIONAL JOURNAL FOR PARASITOLOGY
Volume 31, Issue 14, Pages 1617-1625Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0020-7519(01)00300-9
Keywords
AG5; oesophageal gland cells; nucleotide sequence; plant-parasitic nematode; secretory proteins; cyst nematode
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Secretory proteins encoded by genes expressed in the oesophageal gland cells of plant-parasitic nematodes have key roles in nematode parasitism of plants. Two venom allergen-like protein cDNAs (designated hg-vap-1 and hg-vap-2)were isolated from Heterodera glycines gland cell cDNA libraries. Both cDNAs hybridised to genomic DNA of H. glycines in Southern blots. The hg-vap-1 cDNA contained an open reading frame encoding 215 amino acids with the first 25 amino acids being a putative secretion signal. The hg-vap-2 cDNA contained an open reading frame encoding 212 amino acids with the first 19 amino acids being a putative secretion signal. Genes of hg-vap-1 and hg-vap-2 contained four introns, which ranged in size from 44 to 574 bp, and Eve exons ranging in size from 43 to 279 bp. In situ hybridisation analyses showed that mRNAs of both vap genes. accumulated specifically in the subventral gland cells of H. glycines during parasitism. The gland cell-specific expression and presence of predicted secretion signal peptides in both VAPs suggest that these proteins are secreted from the nematode and may play a role in the infection of host plants by this parasite. (C) 2001 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.
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