Journal
REGULATORY TOXICOLOGY AND PHARMACOLOGY
Volume 34, Issue 3, Pages 274-286Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/rtph.2001.1498
Keywords
allergic contact dermatitis; local lymph node assay; guinea pig; mice
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Funding
- NIEHS NIH HHS [N01-ES-85424] Funding Source: Medline
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To evaluate the reliability of the murine local lymph node assay (LLNA), a test for allergic contact dermatitis activity, the inter- and intralaboratory consistency statistics (h and k, respectively) were calculated for validation studies testing multiple chemicals. The analysis indicated the absence of excessive variability in the dose calculated to induce a threefold or greater increase in the stimulation index (SI). To assess the appropriateness of using an SI of 3 as the decision criteria for identifying a sensitizing compound, LLNA results based on SI values of 2.0,2.5,3.0,3.5, and 4.0 were compared with guinea pig or human results. The results supported the use of an SI of 3 as the decision criteria. Assay performance was determined by comparing LLNA results to results obtained for guinea pigs or humans. The accuracy of the LLNA was 89% when compared with results from the guinea pig maximization test (GPMT)/Buehler assay (BA). The performance of the LLNA and the GPMT/BA was similar when each was compared to human maximization test results plus substances included as human patch test allergens. The LLNA offered advantages over the GPMT in respect to both the time required to conduct the test and the assay cost. (C) 2001 Elsevier Science.
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