Journal
JOURNAL OF ANTIBIOTICS
Volume 54, Issue 12, Pages 1045-1053Publisher
JAPAN ANTIBIOT RES ASSN
DOI: 10.7164/antibiotics.54.1045
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The structure of goadsporin was determined by using spectroscopic techniques. NMR analysis revealed that goadsporin consists of 19 amino acids, two of which are dehydroalanines (Deala), and six of which are cyclized to oxazoles (Oxz) and thiazoles (Thz) by dehydrative cyclization and dehydrogenation from serine, threonine and cysteine. NMR analysis established seven partial structures, and their sequence was determined by CID-MS/MS. Negative mode FAB-MS/MS gave product ions arising from charge-remote fragmentation that allowed determination of the sequence of the amino acid components as AcNH-Ala-MeOxz-Val-DealaMeOxz-Ile-Leu-Thz-Ser-Gly-Gly-MeOxz-Leu-Deala-Oxz-Ala-Gly-Thz-Val-OH, The chiral amino acids were determined by the advanced Marfey's method to have L-configurations.
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