Journal
GENERAL AND COMPARATIVE ENDOCRINOLOGY
Volume 124, Issue 3, Pages 257-268Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/gcen.2001.7710
Keywords
CHH family peptides; jelly layer precursor protein; vitellin; ovarian proteins; penacids
Categories
Ask authors/readers for more resources
Mature penaeid oocytes possess extracellular cortical rods (CR) that contain precursor proteins of the jelly layer (JL) that forms a protective layer around eggs immediately after spawning and dissipates following the assembly of the hatching envelope. The temporal pattern of protein synthesis and mRNA expression of a jelly layer precursor protein in Penaeus semisulcatus ovaries was followed during vitellogenesis, and the regulation by sinus gland extracts (SGE) and crustacean hyperglycemic hormone (CHH) family peptides was evaluated. An similar to33-kDa jelly layer precursor protein was previously identified in ovaries, CR, and JL and was named shrimp ovarian peritrophin-like protein (SOP), because its deduced amino acid sequence shows structural similarities to insect peritrophins. SOP was synthesized in ovarian explant fragments that were removed from vitellogenic ovaries and incubated in vitro, but synthesis was not detected in explants that were collected from previtellogenic ovaries. SOP transcripts were detected in all stages of ovarian development, but were more abundant in previtellogenic ovaries than in other stages. De novo synthesis of SOP was inhibited by P. semisulcatus SGE and by CHH family peptides that were purified from P. japonicus sinus glands. Sinus gland extracts, however, did not affect the steady state levels of SOP transcripts at any stage of ovarian development. These results suggest that SGE regulate SOP synthesis at the posttranscriptional level. (C) 2001 Elsmer Science.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available