Journal
BIOSENSORS & BIOELECTRONICS
Volume 16, Issue 9-12, Pages 1059-1062Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/S0956-5663(01)00227-5
Keywords
surface plasmon resonance; molecular imprinting; sialic acid; ganglioside; biomimetic sensor
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A surface plasmon resonance (SPR) sensor using a molecularly imprinted polymer-coated sensor chip for the detection of sialic acid was developed. The thinly coated polymer was prepared by co-polymerizing N,N,N-trimethylaminoethyl methacrylate, 2-hydroxyethyl methacrylate and ethyleneglycol dimethacrylate in the presence of p-vinylbenzeneboronic acid ester with sialic acid. The sensor showed a selective response to ganglioside of which sialic acid is located at the non-reducing end and gave a linear relationship from 0.1 to 1.0 mg of ganglioside. (C) 2001 Elsevier Science B.V. All rights reserved.
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