Journal
BIOINFORMATICS
Volume 17, Issue 12, Pages 1226-1227Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/17.12.1226
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MELTING computes the enthalpy and entropy of an oligonucleotide duplex helix-coil transition, and then its melting temperature. The program uses the method of nearest-neighbours. The set of thermodynamic parameters can be easily customized. The program provides several correction methods for the concentration of salt. MELTING is a free program, available at no cost and open-source. Perl scripts are provided to show how MELTING can be used to construct more ambitious programs.
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