Journal
METHODS
Volume 25, Issue 4, Pages 402-408Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/meth.2001.1262
Keywords
reverse transcription polymerase chain reaction; quantitative polymerase chain reaction; relative quantification; real-time polymerase chain reaction; Taq Man
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The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control. The 2(-DeltaDeltaCr) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-DeltaDeltaCr) method. In addition, we present the derivation and applications of two variations of the 2(-DeltaDeltaCr) method that may be useful in the analysis of real-time, quantitative PCR data. (C) 2001 Elsevier science.
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