Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 67, Issue 12, Pages 5420-5424Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.67.12.5420-5424.2001
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Funding
- NIEHS NIH HHS [2P30ES00002, P30 ES000002, R01 ES007036] Funding Source: Medline
- NIOSH CDC HHS [R01 OH03489, R01 OH003489] Funding Source: Medline
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(1 -->3)-beta -D-Glucans have been recognized as a potential causative agent responsible for bioacrosol-induced respiratory symptoms observed in both indoor and occupational environments. A specific enzyme immunoassay was developed to quantify (1 -->6) branched, (1 -->3)-beta -D-glucans in environmental samples. The assay was based on the use of a high-affinity receptor (galactosyl ceramide) specific for (1 -->3)-beta -D-glucans as a capture reagent and a monoclonal antibody specific for fungal cell wall P-D-glucans as a detector reagent. The assay, was highly specific for (1 -->6) branched, (1 -->3)-beta -D-glucans (such as that from Saccharomyces cerevisiae) and did not show any response at 200 ng/ml to curdlan, laminarin, pustulan, dextran, mannan, carboxymethyl cellulose, and endotoxins. The detection level was 0.8 ng/ml for baker's yeast glucan and Betafectin. A coefficient of variation of 7.8% was obtained for (1 -->3)-beta -D-glucans in house dust samples. Metal working fluids spiked with (1 -->3)-beta -D-glucans inhibited the glucan assay. Because the assay is specific for (1 -->6) branched, (1 -->3)-beta -D-glucans and is sensitive and reproducible, it will be useful for the investigation of health effects from exposure to this class of biologically active molecules.
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