Journal
FEMS MICROBIOLOGY ECOLOGY
Volume 38, Issue 1, Pages 21-31Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6941.2001.tb00878.x
Keywords
resin acid biodegradation; RNA : DNA ratio; metabolic activity; quantitative RT-PCR; quantitative PCR; wastewater treatment
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Resin acids contribute to the acute toxicity of pulp and paper mill effluent, We monitored the abundance and metabolic activity of the resin acid degrader, Pseudomonas abietaniphila BKME-9, within complex communities of laboratory-scale effluent treatment systems using competitive PCR and competitive RT-PCR, targeting the 16S rDNA and rRNA. In continuous culture, a positive linear relationship was found between the rRNA:rDNA ratio and specific growth rate of BKME-9. In batch culture, the rRNA:rDNA ratio peaked briefly during early exponential growth of BKME-9. During continuous growth of an activated sludge community inoculated with BKME-9, the rRNA:rDNA ratio of BKME-9 decreased when the community was stressed with an alkaline pH shock. After the pH shock, the metabolic activity and population of BKME-9 recovered concomitantly with recovery of resin acid removal activity in the system. Thus, BKME-9 has characteristics that could be useful for bioaugmentation of biotreatment systems, and it is possible to monitor the population and activity of BKME-9 in such complex systems. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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