Journal
JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN
Volume 42, Issue 6, Pages 367-373Publisher
FOOD HYG SOC JPN
DOI: 10.3358/shokueishi.42.367
Keywords
genetic modified; papaya; carotenoid; HPLC
Categories
Ask authors/readers for more resources
We compared the carotenoid profile in GM papaya (Sunup) line to that of a non-GM one (Sunset). First, to identify major carotenoids in papaya, large-scale extraction was carried out with methanol. HPLC analyses of the methanol extracts revealed that both papayas mainly contained 5 pigments and no apparent difference was observed in the HPLC profiles. On the basis of LC/MS data and photodiode-array spectra, beta -carotene (3), lycopene (2), beta -cryptoxanthin (1), and beta -cryptoxanthin myristoyl and lauroyl esters (4 and 5) were identified as major carotenoids. It is well known that most carotenoids are labile, so a rapid analysis with precautions to avoid decomposition was developed to quantify their contents in the original fruits. Frozen samples of the fruits were sliced and a piece (about 2 g) of fruit was cut out and lyophilized. After extraction of the piece with methanol containing an anti-oxidant, BHT, the extract was further partitioned with hexane and methanol. Finally the contents of the main carotenoids in the hexane fraction were analyzed by HPLC. The contents of total carotenoids (sum of 1 similar to5) and beta -cryptoxanthin (1, 4 and 5) in GM papaya fruit were estimated to be 0.764+/-0.056 and 0.604+/-0.051 (mu mol/g), respectively and those in non-GM fruit were 0.883+/-0.145 and 0.705+/-0.098 (mu mol/g), respectively. These differences are not statistically significant.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available