Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 26, Issue 5-6, Pages 929-938Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0731-7085(01)00447-2
Keywords
melatonin; pyridoxine; GC-MS determination; tablets; mass fragmentation
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A gas chroma tographic-mass spectrometric (GC-MS) method for the qualitative and quantitative determination of melatonin plus pyridoxine commercial tablets is described. Melatonin and pyridoxine were simultaneously determined by GC-MS after extraction from ground tablets with methanol and derivatization with N-methyl-N-N-trimethlyisilyltrifluoroacetamide (MSTFA). The mass chromatograms were generated using 232 m/z ion for melatonin and 280 m/z ion for pyridoxine, respectively. Splitless injection offers good reproducibility with a standard deviation of 2%. The developed method was applied to analyze the melatonin and pyridoxine content from two different tablet formulations. Also, recovery, detection and quantification limits are reported. (C) 2001 Elsevier Science B.V. All rights reserved.
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