Journal
ANAEROBE
Volume 7, Issue 6, Pages 297-306Publisher
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
DOI: 10.1006/anae.2001.0399
Keywords
Clostridium sp EG3; dissimilatory Fe(III) reduction; cyclic voltammogram; electrochemically active bacteria; microbial fuel cell
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An obligatory anaerobic bacterium was isolated from a mediator-less microbial fuel cell using starch processing wastewater as the fuel and designated as EG3. The isolate was Gram-positive, motile and rod (2.8-3.0 mum long, 0.5-0.6 mum wide). The partial 16S rRNA gene sequence and analysis of the cellular fatty acids profile suggested that EG3 clusters with Clostridium sub-phylum and exhibited the highest similarity (98%) with Clostridium butyricum. The temperature and pH optimum for growth were 37degreesC and 7.0, respectively. The major products of glucose and glucose/Fe(O)OH metabolism were lactate, formate, butyrate, acetate, CO2 and H-2. Growth was faster at the initial phase and the cell yield was higher when the medium was supplemented with Fe(O)OH than without Fe(O)OH. These results suggest that Fe(III) ion is utilised as an electron sink. Cyclic voltammetry showed that Clostridium butyricum EG3 cells were electrochemically active. It is a novel characteristic of strict anaerobic Gram-positive bacteria. (C) 2001 Academic Press.
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