4.6 Article

A ratiometric colorimetric detection of the folate receptor based on terminal protection of small-molecule-linked DNA

Journal

ANALYST
Volume 140, Issue 4, Pages 1260-1264

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c4an02115k

Keywords

-

Funding

  1. National Natural Science Foundation of China [21371007]
  2. Anhui Provincial Natural Science Foundation [1208085QB28]
  3. Anhui Provincial Natural Science Foundation for Distinguished Youth [1408085J03]
  4. Natural Science Foundation of Anhui [KJ2012A139]
  5. Program for Innovative Research Team at Anhui Normal University

Ask authors/readers for more resources

Development of strategies for the sensitive and selective detection of the folate receptor (FR) that are simple and low cost is of great importance for assessing cancer therapeutics due to its crucial role in physiological, pharmacological and pathological processes. In this paper, gold nanoparticle (AuNP)-based novel ratiometric colorimetry for the detection of the folate receptor (FR) is proposed based on terminal protection of small-molecule-linked DNA. The single-stranded DNA (ssDNA) terminally tethered to folic acid (FA) is protected from degradation by exonuclease I (Exo I) when the FA moiety is bound to FR. The hybridization between FR-protected DNA and DNA-functionalized Au NPs generated a red-to-purple colour change, allowing the visual detection of FR. The detection limit of FR can be as low as 0.33 ng mL(-1) with the naked eye. It provides a promising strategy for visual detection of the binding event of FA to its protein receptor-FR with advantages such as simplicity, high selectivity, and a wide linear range.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available