Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 67, Issue 12, Pages 5608-5613Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.67.12.5608-5613.2001
Keywords
-
Categories
Funding
- NIGMS NIH HHS [5RO1GM60052-02, R01 GM060052] Funding Source: Medline
Ask authors/readers for more resources
Detachment from biofilms is an important consideration in the dissemination of infection and the contamination of industrial systems but is the least-studied biofilm process. By using digital time-lapse microscopy and biofilm flow cells, we visualized localized growth and detachment of discrete cell clusters in mature mixed-species biofilms growing under steady conditions in turbulent How in situ. The detaching biomass ranged from single cells to an aggregate with a diameter of approximately 500 mum. Direct evidence of local cell cluster detachment from the biofilms was supported by microscopic examination of filtered effluent. Single cells and small clusters detached more frequently, but larger aggregates contained a disproportionately high fraction of total detached biomass. These results have significance in the establishment of an infectious dose and public health risk assessment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available