Journal
METHODS
Volume 25, Issue 4, Pages 456-462Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/meth.2001.1268
Keywords
CpG island; hypermethylation; DNA methylation; Taqman; real-time PCR; sodium bisulfite
Funding
- NCI NIH HHS [R01 CA075090] Funding Source: Medline
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MethyLight is a sensitive, fluorescence-based real-time PCR technique that is capable of quantitating DNA methylation at a particular locus by using DNA oligonucleotides that anneal differentially to bisulfite-converted DNA according to the methylation status In the original genomic DNA. The use of three oligonucleotides (forward and reverse primers, and interpositioned probe) in MethyLight, any one or more of which can be used for methylation discrimination, allows for a high degree of specificity, sensitivity, and flexibility In methylation detection. (C) 2001 Elsevier Science (USA).
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