Journal
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
Volume 70, Issue 4, Pages 457-466Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0091-3057(01)00700-6
Keywords
acetylcholine; axotomy; brainstem; hypothalamus; motoneurons; nicotinic antagonists; reconstituted receptors; whole-cell recordings
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The properties of nicotinic acetylcholine receptors (nAChRs) were studied following exogenous expression in a host system or using whole-cell recordings in brain slices, autoradiography and immunohistochemistry. When expressed in HEK-293 cells, alpha4beta2 nAChRs displayed both a high and a low affinity component. The ratio of these two states was modified by chronic nicotine exposure, resulting in an enhanced sensitivity and a marked reduction in desensitization. Mutations in the gene coding for the alpha4 subunit are responsible for a particular form of nocturnal epilepsy. When expressed in Xenopus oocytes, alpha4beta2 nAChRs containing these mutations displayed distinct alterations in agonist affinity, desensitization and calcium permeability. Magnocellular endocrine neurons in the supraoptic (SO) nucleus of the hypothalamus were found to express functional alpha7-containing nAChRs, which could play a role in regulating neurohypophysial peptide secretion. Facial (VII), hypoglossal (XII) and vagal (X) motoneurons of young rats responded to ACh by a fast inward current. The nAChRs present in VII and XII nuclei were of the non-alpha7-containing type, whereas those present in the X nucleus contained the alpha7 subunit. In Bcl-2 transgenic mice, facial nerve axotomy caused nAChRs downregulation by interfering negatively with the expression of the alpha4 subunit. Binding sites corresponding to alpha7-containing nAChRs were also detected in spinal motor nuclei and axotomy provoked a reduction of the binding. Together, these data indicate that long-term exposure to nicotine can promote neuroadaptive changes in nAChRs and that genetic alterations of neuronal nAChRs can result in transmissible neurological diseases. They also suggest that these receptors probably play a role in the central regulation of autonomic functions, as well as in motor control. (C) 2001 Elsevier Science Inc. All rights reserved.
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