Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 24, Issue 4, Pages 554-560Publisher
ELSEVIER GMBH
DOI: 10.1078/0723-2020-00071
Keywords
Lactobacillus plantarum group; RAPD-PCR; AFLP; genomic relationship; taxonomy
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Two high-resolution genotypic techniques (RAPD-PCR and AFLP) were evaluated for their possibility to discriminate the species Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus paraplantarum and to type these taxa at the infra-species level. In total 23 strains of L. plantarum, three strains of L. pentosus, two strains of L. paraplantarum and two related strains for which the species assignment was not clear, were studied. For RAPD-PCR, suitable oligonucleotides and amplification conditions were selected and tested. For AFLP, a double digest of total genomic DNA was used and a subset of restriction fragments was selectively amplified and visualised using different primer combinations. Both methodologies generated species-specific electrophoretic profiles. Moreover, the presence of distinct Subgroups was revealed within the species L. plantarum.
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