Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 258, Issue 1-2, Pages 183-191Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0022-1759(01)00474-4
Keywords
flow cytometry; antibody-dependent cell-mediated cytotoxicity PKH-26; TO-PRO-3 iodide
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Using flow cytometry (FCM), an assay has been developed for the determination of antibody-dependent cell-mediated cytotoxicity (ADCC). Target cells were labelled with a membrane dye, PKH-26, to allow discrimination when incubated with effector cells and antibody. Post-incubation, cell death within the PKH-26(+) target cell population was assessed by the addition of the viability probe TO-PRO-3 iodide (TP3). This ADCC method allows analysis to be conducted on a single cell basis and overcomes the need for radiochemicals. This communication indicates that the assay is accurate and reproducible with the potential to be a useful tool for evaluating the therapeutic potential of antibodies and antibody-based reagents. (C) 2001 Elsevier Science B.V. All rights reserved.
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