Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 92, Issue 6, Pages 590-593Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1263/jbb.92.590
Keywords
liposome; green fluorescent protein; cell-free protein synthesis; flow cytometry; fluorescence microscopy
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The liposome consisting of eggPC, cholesterol, and DSPE-PEG5000 with a molar ratio of 1.5:1:0.08 was used to entrap cell-free protein synthesis reaction mixture. The synthesis of a mutant green fluorescent protein in the liposome was confirmed by the fluorescence emitted from the liposome on flow cytometry analysis and fluorescence microscopy. The protein synthesized in the liposome is hence functional.
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