Journal
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 71, Issue 1, Pages 101-104Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-1605(01)00566-9
Keywords
Escherichia coli; high hydrostatic pressure; membrane damage; sublethal injury
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Cells of Escherichia coli that survived pressure treatment at 400 MPa showed increased sensitivity to sodium deoxycholate or sodium chloride in the plating medium, implying that homeostatic or barrier functions associated with outer and cytoplasmic membranes, respectively, were impaired. Repair of such sublethal membrane damage occurred when cells were incubated at 37 degreesC in tryptone soya broth. Inhibitor studies indicated that repair of cytoplasmic membrane damage was energy-dependent and required RNA and protein synthesis, whereas repair of outer membrane damage occurred with no requirement for energy or RNA or protein synthesis. (C) 2001 Elsevier Science B.V. All rights reserved.
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