Journal
FEBS LETTERS
Volume 509, Issue 3, Pages 435-438Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(01)03206-9
Keywords
reactive oxygen species; cytochrome c; cardiolipin; apoptosis
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Cytochrome c release from mitochondria is a critical event in the apoptosis induction. Dissociation of cytochrome c from the mitochondrial inner membrane (IMM) is a necessary first step for cytochrome c release. In the present study, the effect of reactive oxygen species (ROS) on the dissociation of cytochrome c from beef-heart submitochondrial particles (SMP) and on the cardiolipin content was investigated. Exposure of SNIP to mitochondrial-mediated ROS generation resulted in a large dissociation of cytochrome c from SMP and in a parallel loss of cardiolipin. Both these effects were directly and significantly correlated and also abolished by superoxide dismutase+catalase. These results demonstrate that ROS generation induces the dissociation of cytochrome c from IMM via cardiolipin peroxidation. The data may prove useful in clarifying the molecular mechanism underlying the release of cytochrome c from the mitochondria to the cytosol. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
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