STR primer concordance study

Over 1500 population database samples comprising African Americans, Caucasians, Hispanics, Native Americans, Chamorros and Filipinos were typed using the PowerPlex(R) 16 and the Profiler Plus(TM)/COfiler(TM) kits. Except for the D8S1179 locus in Chamorros and Filipinos from Guam, there were eight examples in which a typing difference due to allele dropout was observed. At the D8S1179 locus in the population samples from Guam, there were 13 examples of allele dropout TM observed when using the Profiler Plus kit. The data support that the primers used in the PowerPlex(R) 16, Profiler Plus, and COfiler(TM) kits are reliable for typing reference samples that are for use in CODIS. In addition, allele frequency databases have been established for the STR loci Penta D and Penta E. Both loci are highly polymorphic. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.