Journal
GENES & DEVELOPMENT
Volume 15, Issue 24, Pages 3237-3242Publisher
COLD SPRING HARBOR LAB PRESS
DOI: 10.1101/gad.946401
Keywords
homologous recombination; DNA double-strand break; nonhomologous end-joining; Ku protein; DNA-PK; sister-chromatid exchange; gene targeting
Categories
Funding
- NCI NIH HHS [R01 CA085867, R01 CA085867-01A2] Funding Source: Medline
- NIGMS NIH HHS [GM54688] Funding Source: Medline
Ask authors/readers for more resources
Chromosomal double-strand breaks (DSBs) in mammalian cells are repaired by either homology-directed repair (HDR), using a homologous sequence as a repair template, or nonhomologous end-joining (NHEJ), which often involves sequence alterations at the DSB site. To characterize the interrelationship of these two pathways, we analyzed HDR of a DSB in cells deficient for NHEJ components. We find that the HDR frequency is enhanced in Ku70(-/-), XRCC4(-/-), and DNA-PKcs(-/-) cells, with the increase being particularly striking in Ku70(-/-) cells. Neither sister-chromatid exchange nor gene-targeting frequencies show a dependence on these NHEJ proteins. A Ru-modulated two-ended versus one-ended chromosome break model is presented to explain these results.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available